The following bytes were changed (at minimum) from the original ROM (checksum ????????):

0000008D: Changed space after "3.1 ROM" to NULL (why?)
00000511-00000513: Changed to 0x27FF0A (jump to cache clear code?)
000035DC-000035DD: Changed to 0x4A2E to disable beta alert
         0004D821: Changed to 0xEB to activate NoClick
0007F6F6-0007F71F: Changed Workbench title to "OS 3.1   %lD graphics mem  %lD other mem   "
0007FF00-0007FF4B: Inserted the following block of code (clear caches?):

4C DF 41 01 4E F9 00 20 0A 90 48 E7 80 82 2C 78
00 04 08 2E 00 00 01 29 67 E6 20 6F 00 0E 30 10
02 40 FF C0 0C 40 40 C0 66 D6 00 10 00 02 08 2E
00 01 01 29 67 10 4E 7B 88 02 4E 7A 00 02 08 C0
00 02 4E 7B 00 02 4C DF 41 01 4E 73

0007FFE8-0007FFEB: Changed ROM checksum to 356714E3